Journal: Drug Delivery

Article Title: 3D optical/CT as a preclinical companion imaging platform for glioblastoma drug development

doi: 10.1080/10717544.2020.1833381

Figure Lengend Snippet: Validation of 3D optical/CT localization of Cy7 labeled mouse albumin (Cy7ALB) directly injected into the brain of a normal mouse. 5 µl of Cy7ALB was injected into right hemisphere. ( A ) Cy7 epi fluorescent images of 2 different mice 24 h after intracranial injection of Cy7ALB showing accumulation on 2D (left) and 3D OI/CT (right), confirming intracranial placement of infused Cy7ALB in the top mouse but superficial localization in the bottom mouse. Note that the intensity on the epi fluorescent images is heavily influenced by how close to the surface the dye is located rather than amount of Cy7ALB present, due to tissue attenuation of the Cy7 signal. (B) Confirmation of localization of Cy7ALB 3D OI/CT on sections of mouse brain imaged post necropsy. Mice were implanted with Cy7ALB at 2.5 mm depth (top, n = 3) and 1 mm depth (bottom, n = 3) and imaged with 3D OI/CT 24 h after injection (left panels). Immediately after imaging, mice were euthanized and their brains were sectioned and imaged (right panels) to confirm localization of 3D OI/CT images. (C) Coronal 3D OI/CT images of a representative mouse 24 h (top) and 96 h (bottom) after injection of Cy7ALB signal, demonstrating intracranial signal 24 h after injection but not at 96 h, indicating the potential to reimage subsequent infusions. Quantification of the results is shown on the right ( n = 4).

Article Snippet: The 3D fluorescent image was acquired under isoflurane anesthesia using MILabs 3D OI/CT scanner 24 h after tracer administrations.

Techniques: Labeling, Injection, Imaging

Journal: Drug Delivery

Article Title: 3D optical/CT as a preclinical companion imaging platform for glioblastoma drug development

doi: 10.1080/10717544.2020.1833381

Figure Lengend Snippet: 3D optical/CT visualization of glioblastoma in a mouse brain using systemically injected Cy7ALB. (A) Bioluminescence (biolum) image of mouse with implanted G48a-FLuc cells. (B) Coronal (top) and sagittal (bottom) views of microCT with Omnipaque TM contrast (left panels) and 3D optical/CT (right panels) 24 h post systemic injection of Cy7ALB. Images demonstrate intracranial Cy7ALB accumulation in the mouse with growing G48a GBM caused by BBB permeability in the region of the tumor, corresponding to the CT contrast accumulation. (C) Tumor to background ratio obtained using Cy7-albumin 3D optical detection (Cy7ALB) and CT with contrast (CT). (D) After imaging, mice were injected with Evan’s blue (EB) dye and euthanized 4 h later to confirm BBB penetration. Photograph of EB and epi fluorescent images of the post mortem brain of a control non tumor bearing mouse (top) along with brain of the GBM-bearing mouse from images A and B (bottom), confirming BBB permeability and Cy7ALB accumulation, respectively.

Article Snippet: The 3D fluorescent image was acquired under isoflurane anesthesia using MILabs 3D OI/CT scanner 24 h after tracer administrations.

Techniques: Injection, Permeability, Imaging

Journal: Regenerative Biomaterials

Article Title: Thermosensitive hydrogel with programmed dual-octenidine release combating biofilm for the treatment of apical periodontitis

doi: 10.1093/rb/rbae031

Figure Lengend Snippet: Evaluation of periapical inflammation after using intracanal medicaments of infected root canals in vivo . ( A ) Micro-CT and 3D reconstruction images of periapical inflammation in blank, CHX gel, Ca(OH) 2 paste and OCT/PECT@OCT + ALK gel groups. ( B ) Image of periapical lesion size (mm 3 ). Statistic were analysed by CTvox and SPSS 25.0. Statistical significance is indicated as * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Their teeth along with bilateral maxillae were removed for micro-CT analysis and 3D reconstruction by using CTvox (Bruker, sky scan 1276, Germany) to examine the healing of periapical inflammation ( ).

Techniques: Infection, In Vivo, Micro-CT